Spermidine

Spermidine is a naturally occurring polyamine with three amino groups distributed along a flexible carbon chain. Its protonated amines interact with negatively charged nucleic acids, while its participation in cellular polyamine pathways makes it a useful reagent for studies of growth, stress responses and autophagy. These chemical and biological functions underpin distinct laboratory applications.

The selected material is the free base for molecular-biology and cell-research use. In nucleic-acid workflows, spermidine is used in certain transcription buffers and in precipitation procedures for DNA. In cell experiments, it is supplied as an exogenous polyamine to investigate regulatory pathways and autophagic responses. The free-base identity is important for preparing molar solutions, because hydrochloride salts have different formula weights and counterion contents.

Typical Applications

• In-vitro transcription systems: Used as a polyamine component of established transcription-buffer formulations to support the nucleic-acid reaction environment.
• DNA precipitation workflows: Used in procedures that exploit polyamine–DNA interactions to precipitate and recover nucleic acids, including fragments longer than approximately 100 base pairs in reported protocols.
• Cellular polyamine and autophagy research: Used in cultured-cell experiments to investigate responses to added spermidine, including changes in autophagic activity and polyamine-dependent pathways.

Specifications

Specifications describe the standard grade. Tolerance, test method and documentation package are confirmed per order.