Glucose oxidase enzyme preparation
CAS No.
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Molecular formula
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Chemical Product Description & Applications
Glucose oxidase catalyzes oxidation of glucose using molecular oxygen, producing gluconolactone and hydrogen peroxide. In dough, the generated peroxide supports oxidative strengthening reactions in the gluten network. This can improve resistance to mechanical stress and help the dough retain fermentation gas during breadmaking.
The selected material is a commercial baking-enzyme preparation rather than a purified analytical reagent. Its function depends on available glucose and oxygen, so activity in dough follows the surrounding formulation and processing conditions. Stronger gluten structure can improve handling and reduce stickiness, while gas retention supports loaf development. The enzyme acts catalytically; it is not a bulk gluten replacement or a direct source of leavening gas.
Typical Applications
- Bread improver blends: Used as the oxidative enzyme component in formulated improvers intended to strengthen dough and improve processing tolerance.
- Industrial pan-bread doughs: Used in appropriate breadmaking systems to support gluten-network stability, gas retention and loaf development.
- Flour-correction systems: Used in compatible enzyme packages where weaker flour requires improved dough strength and handling consistency.
Specifications
| Parameter | Specification | Test Method |
|---|---|---|
| Appearance | Cream to tan powder | Visual inspection |
| Identification | Protein fingerprint and stated enzyme activity match reference | Protein electrophoresis plus substrate activity assay |
| Enzyme activity | ≥10000 U/g | Glucose oxidation at pH 5.1, 35 C; 1 U oxidizes 1 micromole glucose/min |
| Loss on drying | ≤8.0% w/w | Vacuum-oven gravimetry |
| Lead | ≤5 mg/kg | ICP-MS after digestion |
| Salmonella | Absent in 25 g | Enrichment and culture confirmation |
Specifications describe the standard grade. Tolerance, test method and documentation package are confirmed per order.
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