| Appearance |
Cream to tan powder |
Visual inspection |
| Identification |
Protein fingerprint and stated enzyme activity match reference |
Protein electrophoresis plus substrate activity assay |
| Keratinolytic activity |
≥10000 U/g |
Keratin hydrolysis, pH 8.0, 50 C; 1 U releases 1 microgram tyrosine equivalents/min |
| Loss on drying |
≤8.0% w/w |
Gravimetric vacuum drying |
| Aerobic bacterial count |
≤50000 CFU/g |
Plate count with enzyme neutralization |
| Salmonella |
Absent in 25 g |
Enrichment and culture confirmation |